Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 19 - Jul 24
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Rat TFAM ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Transcription Factor A, Mitochondrial (TFAM) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Transcription Factor A, Mitochondrial (TFAM) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Transcription Factor A, Mitochondrial ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Mitochondrial transcription factor A (TFAM) is a protein encoded by the TFAM gene. This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription and a participant in mitochondrial genome replication. It binds to mitochondrial promoter DNA to facilitate mitochondrial genome transcription. It is a double-box, high-mobility group DNA binding and bending protein. This bending function is important for mitochondrial transcription initiation in mammals, but not in its yeast homolog, Abf2. It may also participate in mitochondrial genome packaging, as its binding activity is non-sequence-specific. It has been shown to interact with TFB1M and TFB2M. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 16 reviews
Sort
Product Reviews
★★★★★ 5
Saatva Pillow is great
Size: Standard/Queen (Pack of 1), Style: Standard Loft (4" - 5")
So far this is the best pillow I have had in the last 10 years and I have tried two Purple pillows a couple different memory foam and Gel pillows at least 4 more pillows that I can't even remember the names of. My neck and shoulders are almost pain free. I have also been doing more stretching of my arms which is also helping, but this pillow has really made the most difference in the pain I have had, I felt difference from the first time I put my head on it. It's comfortable, the perfect height I got the 4"-5", I also got the queen size and it's great. It supports my head just right. I also ordered the Saatva Classic 14" mattress from the company and I sure hope it's as good or better would be great because I have back issues for the last 50 years.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 16, 2025
★★★★★ 1
Cotton Fluff Around a Mostly Empty Bag
Size: King (Pack of 1), Style: High Loft (6"-7")
Bought the high-loft king, thinking it would be a great (tall) side-sleeper. What I got? A very nice, completely compressible cotton outer shell wrapped around a bag a bit smaller than a standard pillow. In that bag? A tiny amount of small latex chunks, just about a third full. That’s right, some cotton fluff around a basically empty bag. Could you buy some latex yourself and add it? No, the bag is sewn shut. You could probably fill a small pillow with latex you bought elsewhere and put that in. But seriously, what did you pay Saatva for in that case?
Probably as close as I’ve seen a major/high-reputation company get towards a literal example of rip off.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 31, 2026
★★★★★ 3
Wrong item ships/decent pillow/price unjustified
Size: Standard/Queen (Pack of 1), Style: Standard Loft (4" - 5")
Sent me the wrong pillow twice in a row. It said high loft and standard loft on the packing, not sure what the problem is. I ordered standard loft both times, and both times they sent high loft. That name is very deceiving though. If what I have is high loft, the standard loft wouldn’t be much thicker than your standard flat down pillow. If you’d like any sort of neck support I’d suggest starting with the high loft and going down instead of the other way around. I am 5’ 7” 130lbs and this pillow does not lift my neck up further than neutral, which is a good thing. Shredded memory foam pillows are significantly higher or thicker if that helps.
Pillow itself grew on me. You can definitely feel that there is a different material in the center of the pillow. I ordered it as a gift and ended up keeping both pillows. The price tag on this item is absolutely outrageous. I would have never ordered it for myself. Saatva products don’t last in my experience, we got their firmest mattress and within 2 years it was sagging horribly. I am interested to see how long this pillow lasts!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 19, 2026
★★★★★ 2
Side sleeper- lost loft within 2 weeks
Size: Standard/Queen (Pack of 1), Style: High Loft (6"-7")
Side sleeper and within 2 weeks this pillow has lost its loft and has become lumpy. I have spent so much money trying to support my neck and shoulders, this unfortunately is not it. I will continue my search and return this one.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 26, 2026
★★★★★ 4
Mixed Feelings on the Saatva Pillow - A Comprehensive Review
Size: King (Pack of 1), Style: High Loft (6"-7")
Updated Edit (June 2024) : In my quest to find the perfect pillow, I initially purchased the high loft version of this pillow, but it turned out to be a bit too high for my liking. I then decided to try the standard size, and I like it much better! The pillow can still get lumpy and requires fluffing, but opting for the standard lower loft has definitely improved my experience with it. While I still use this pillow occasionally, I generally stick with my Purple Harmony pillow more. Changing my rating to 4 stars.
---
Background:
Over the past 2 years, I have embarked on a journey to find the perfect pillow, testing over ten different options, including numerous high-end brands. My latest trial was the Saatva pillow, renowned for its rave reviews.
Expectations vs. Reality:
Despite its widespread acclaim, my experience with the Saatva pillow did not meet my expectations. I opted for the higher loft variant, aligning with my general preference, yet I found it too high of a lot even as a primary side sleeper.
Material and Comfort:
The Saatva pillow, composed of both latex and down alternative, presented a lumpy texture. This inconsistency resulted in varied support depending on the position of my head. Unlike my regular down alternative pillow, the Saatva required frequent re-fluffing and adjusting throughout the night.
Comparison and Value:
Considering its price and the overwhelming number of positive reviews, my disappointment was notable. Surprisingly, a more affordable down alternative pillow from Amazon outperformed the Saatva in terms of support and comfort, despite the latter's use of higher quality fabrics and its significantly higher cost.
Final Verdict:
This pillow was one of the last in my quest to discover the "best" pillow. Sadly, it ranks in the lower ranks among the high-end, expensive pillows I've tried. I preferred the Purple Harmony pillow over the Saatva, albeit with reservations about the Purple Harmony as well.
Conclusion:
The Saatva pillow, while undoubtedly high-quality and possessing comfortable aspects, did not live up to my expectations, landing it in my pile of unused pillows. It may suit others, but for me, I only give it a 3 out of 5 stars rating.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 26, 2023